Application Notes sorted by Assays
Molecular Biology
Measurement of DNA, ATP, Protein
| [211 - 2.700] |
| Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus |
| Ferneyhough B, Parkinson N |
| BMG LABTECH 2011 |
| Nucleic acid quantification on the PHERAstar Plus microplate reader in fluorescence mode in 384-well format. |
| Article |
| [001 - 6.330] |
| DNA measurements in low volume samples, microplates and cuvettes on the SPECTROstar Nano |
| Groene M, Ganscke F, Wark C, Dell EJ |
| BMG LABTECH 2011 |
| Three different ways to measure DNA samples in the SPECTROstar Nano are in detail explained and advantages of each option as well as sensitivity data are presented. |
| Article |
| [213 - 2.720] |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Miriam Uppill, Antony Sheehan and Ron Osmond |
| BMG LABTECH 2011 |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega |
| Article |
| [202 - 4.340] |
| Promega's ADP-Glo(TM) Kinase Assay performed on BMG LABTECH's PHERAstar FS and POLARstar Omega |
| Ganske, F, Dell, EJ, Shultz S |
| BMG LABTECH 2009 |
| A homogeneous luminescent assay from Promega to detect ADP |
| Article |
| [201 - 2.660] |
| The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers |
| Franka Ganske, BMG LABTECH, Offenburg, Germany; EJ Dell, BMG LABTECH, NC, USA / Meera Kumar, BellBrook Labs, Madison, USA |
| BMG LABTECH 2009 |
| The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay |
| Article |
| [196 - 2.620] |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Muller RE, Siderovski DP, Kimple AJ, Dell EJ |
| BMG LABTECH 2009 |
| Alternative, non-radiological method to assess heterotrimeric G protein activation |
| Article |
| [190 - 7.130] |
| Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus |
| Franka Ganske and Keith Ansell |
| BMG LABTECH 2009 |
| Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented. |
| Article |
| [183 - 1.180] |
| New Transcreener® ADP2 FP Assay performed on BMG LABTECH's POLARstar Omega |
| Larson B, Ganske F, Dell EJ |
| BMG LABTECH 2008 |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| Article |
| [182 - 1.170] |
| New Transcreener® ADP2 FP Assay performed on BMG LABTECH's PHERAstar Plus HTS Microplate Reader |
| Larson B, Ganske F, Dell EJ |
| BMG LABTECH 2008 |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| Article |
| [180 - 1.160] |
| High-throughput protein-DNA affinity measurements using fluorescence anisotropy |
| Veprintsev DB |
| BMG LABTECH 2008 |
| The effect of modified nucleotides on the recognition of DNA by the tumour suppressor protein p53 was studied. |
| Article |
| [176 - 2.570] |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| Olaf Piepenburg and Niall A. Armes |
| BMG LABTECH 2008 |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| Article |
| [170 - 6.210] |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| Dell EJ |
| BMG LABTECH 2008 |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| Article |
| [169 - 6.200] |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Dell EJ and Ganske F |
| BMG LABTECH 2008 |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| Article |
| [168 - 6.190] |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| Ganske F |
| BMG LABTECH 2008 |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| Article |
| [166 - 2.540] |
| Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader |
| Tracey Madgett, University of the West of England, Bristol |
| BMG LABTECH 2008 |
| Protein determination using a fluorescent assay from Invitrogen |
| Article |
| [164 - 2.530] |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| Sarah Shammas and Ann-Christin Brorsson |
| Department of Chemistry and Cavendish Laboratory, University of Cambridge |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| Article |
| [162 - 2.520] |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Danielle M. Corgliano, Heike Hofstetter and Oliver Hofstetter |
| Northern Illinois University, DeKalb, IL, USA |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| Article |
| [152 - 1.140] |
| Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar |
| Ganske F, Dell EJ, Larson B |
| BMG LABTECH 2008 |
| Far-red competitive FP immunoassay for ATP depletion measurements |
| Article |
| [158 - 6.170] |
| Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software |
| Ganske F, Dell EJ |
| BMG LABTECH 2007 |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| Article |
| [135 - 2.430] |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Poon S, Rivers RC |
| Department of Chemistry, University of Cambridge, UK. |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| Article |
| [133 - 5.110] |
| Measurement of Rheumatoid Factor by NEPHELOstar Microplate Reader |
| Mack L, Barton A |
| BMG LABTECH 2005 |
| Detection and quantification of rheumatoid factors (immunoglobulins Igs) |
| Article |
| [128 - 2.410] |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Aucamp J, Cosme AM, Lye GJ, Dalby PA |
| BMG LABTECH 2005 |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
| Article |
| [103 - 2.018] |
| Molecular Probes PicoGreen assay performed on BMG LABTECH POLARstar OPTIMA microplate reader |
| Angersbach S, Earp R. |
| BMG LABTECH |
| A standard DNA quantification assay using fluorescence |
| Article |
| [110 - 4.012] |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| Earp RL, Cheek J |
| BMG LABTECH |
| The ATP detection kit is linear through-out kit range |
| Article |
| [116 - 6.002] |
| DNA Quantification (Absorbance Mode) |
| BMG LABTECH, Germany |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| Article |
| [113 - 4.007] |
| The use of an ATP bioluminescence assay to quantify cell cytotoxicity |
| Hipler B. |
| BMG LABTECH |
| Classical ATP cell cytotoxicity assay based on firefly activity |
| Article |
High Throughput Screening
GPCR (G-Protein Coupled Receptors)
| [229 - 4.390] |
| Nano High-Throughput Screening (nHTS) Platform - Miniaturization of Cell-based GPCR and Kinase Assays |
| 1 E.J. Dell, BMG LABTECH (North Carolina, US) Sunitha Sastry , DiscoveRx (Freemont, US); Bonnie Edwards and Jing Wang, Labcyte Inc (Sunnyvale, US) |
| BMG LABTECH 2012 |
| PHERAstar FS measures chemiluminescence-based screening assays from Discoverx in 1536 and 3456-well plate format |
| Application Note PDF |
| Article |
| [227 - 2.760] |
| Quantifying Fluorescent Ligand Binding to GPCRs in Live Cells using the PHERAstar FS - a new format for HTS |
| Carter A, Thompson K, Wark C |
| BMG LABTECH 2012 |
| Fluorescent dopamine and adenosine ligand binding assays in living cells were performed on the PHERAstar FS using the focal height adjustment and bottom reading mode. |
| Article |
| [222 - 3.230] |
| HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway |
| Dell EJ, Tardieu JL, Degorce F |
| BMG LABTECH 2012 |
| Several low affinity drug hits were only found by the PMT-based PHERAstar FS microplate reader and not with a CCD-based instrument from a different vendor |
| Article |
| [221 - 4.370] |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| K. Atze, B. Ortmann, S. Valentin, M. Kühn, S. Franke, N. Breuer, P. Stecha, B. Binkowski, L. de Bruin, A. Pitt |
| BMG LABTECH 2012 |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| Article |
| [210 - 7.140] |
| An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on BMG LABTECH's POLARstar Omega |
| J.D. Brown, R. Mould and C.J. Langmead |
| BMG LABTECH 2011 |
| POLARstar Omega is used to detect ERK phosphorylation using an AlphaScreen® SureFire® proximity assay. |
| Article |
| [209 - 3.200] |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex" Cell-based Assay |
| Jacquemart L, Dell EJ |
| BMG LABTECH 2010 |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection |
| Article |
| [206 - 2.680] |
| Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader |
| Mark Koeppel, Invitrogen, Madison Wisconsin, USA; EJ Dell, BMG LABTECH, NC, USA |
| BMG LABTECH 2010 |
| Two fluorescent cell-based assays from Invitrogen performed on the PHERAstar FS with direct bottom reading and high resolution well scanning |
| Article |
| [194 - 8.150] |
| FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction |
| Hutsell SQ, Kimple AJ, Siderovski DP |
| BMG LABTECH 2009 |
| CFP-YFP FRET based assay utilized to screen for potential inhibitors |
| Article |
| [196 - 2.620] |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Muller RE, Siderovski DP, Kimple AJ, Dell EJ |
| BMG LABTECH 2009 |
| Alternative, non-radiological method to assess heterotrimeric G protein activation |
| Article |
| [193 - 3.190] |
| Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH |
| Bernd Laffert and Franka Ganske |
| BMG LABTECH |
| HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany. |
| Article |
| [157 - 2.500] |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| Liao J, Dell E.J. |
| ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007 |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| Article |
| [143 - 4.230] |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Rainard JM, Mireylees SE, Darlison MG |
| BMG LABTECH 2006 |
| Luminescence-based assay to detect low levels of cAMP |
| Article |
| [142 - 1.030] |
| HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar |
| Kauffman L, De La Llera, Angersbach S, Orban M |
| BMG LABTECH 2006 |
| A fluorescence polarization based assay from DiscoveRx to measure inositol-3 phosphate levels |
| Article |
| [134 - 4.200] |
| New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar |
| Kauffman L, De La Llera S, Orban M |
| BMG LABTECH 2006 |
| Directly assess either Gi or Gs coupled GPCRs in cells in up to 1536-well format |
| Article |
| [107 - 2.013] |
| Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader |
| Hoefgen B, Kassack MU. |
| Calcium measurements of Oregon Green loaded HEK293 cells |
| Application Note PDF |
Kinases, Cytochrome P450 and Oxygenase
| [234 - 2.780] |
| Real-time fluorescence assay for monitoring transglutaminase activity |
| Magdalena Adamczyk, Andreas Heil and Daniel Aeschlimann Matrix Biology & Tissue Repair Research Unit, School of Dentistry, Cardiff University, Heath Park, Cardiff, CF14 4XY, UK |
| BMG LABTECH 2012 |
| Kinetic fluorescence measurements done on Omega and OPTIMA readers to determine transglutaminase activity |
| Application Note PDF |
| Article |
| [235 - 2.790] |
| Three assays in one well: Antimalarial compound library screening using the FLUOstar Omega |
| Sheena McGowan, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia |
| Kinetic absorbance and fluorescence assays developed to identify inhibitors for metalloaminopeptidases M1, M17 and M18 |
| Application Note PDF |
| Article |
| [229 - 4.390] |
| Nano High-Throughput Screening (nHTS) Platform - Miniaturization of Cell-based GPCR and Kinase Assays |
| 1 E.J. Dell, BMG LABTECH (North Carolina, US) Sunitha Sastry , DiscoveRx (Freemont, US); Bonnie Edwards and Jing Wang, Labcyte Inc (Sunnyvale, US) |
| BMG LABTECH 2012 |
| PHERAstar FS measures chemiluminescence-based screening assays from Discoverx in 1536 and 3456-well plate format |
| Application Note PDF |
| Article |
| [225 - 1.200] |
| Methyltransferase, Acetyltransferase, Kinases, and GTPases Can All Be Measured with Transcreener® Assays |
| Kumar M, Zielinski T, Koop A, Ganske F, Dell EJ |
| BMG LABTECH 2012 |
| All four Transcreener FP assays for nucleotide detection show excellent Z´values and a large signal window on the PHERAstar |
| Article |
| [224 - 2.750] |
| Assessing pancreatic trypsin activity using the POLARstar Omega Microplate Reader |
| W. Huang, M. C. Cane, D. Latawiec, M. Awais, J. Armstrong, M. Chvanov, D. N. Criddle, R. Sutton |
| BMG LABTECH 2012 |
| Fluorimetric kinetic trypsin activity assay performed on a POLARstar Omega as a measure for acute pancreatitis |
| Article |
| [220 - 2.740] |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Haus P and Meyer-Almes FJ |
| BMG LABTECH 2012 |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader from BMG LABTECH. |
| Article |
| [202 - 4.340] |
| Promega's ADP-Glo(TM) Kinase Assay performed on BMG LABTECH's PHERAstar FS and POLARstar Omega |
| Ganske, F, Dell, EJ, Shultz S |
| BMG LABTECH 2009 |
| A homogeneous luminescent assay from Promega to detect ADP |
| Article |
| [203 - 2.650] |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| George Cathcart, Brendan Gilmore, Brian Walker. |
| BMG LABTECH 2009 |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors |
| Article |
| [201 - 2.660] |
| The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers |
| Franka Ganske, BMG LABTECH, Offenburg, Germany; EJ Dell, BMG LABTECH, NC, USA / Meera Kumar, BellBrook Labs, Madison, USA |
| BMG LABTECH 2009 |
| The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay |
| Article |
| [185 - 6.250] |
| Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA |
| Franka Ganske |
| BMG LABTECH 2009 |
| Esterase activity was determined using the p-nitrophenyl acetate (pNPA) assay. Km and Vmax were calculated using different calculation methods (e.g. Michaelis-Menten and Lineweaver-Burk). |
| Article |
| [183 - 1.180] |
| New Transcreener® ADP2 FP Assay performed on BMG LABTECH's POLARstar Omega |
| Larson B, Ganske F, Dell EJ |
| BMG LABTECH 2008 |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| Article |
| [182 - 1.170] |
| New Transcreener® ADP2 FP Assay performed on BMG LABTECH's PHERAstar Plus HTS Microplate Reader |
| Larson B, Ganske F, Dell EJ |
| BMG LABTECH 2008 |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| Article |
| [186 - 7.120] |
| AlphaScreen® Phosphotyrosine Assay Performed on a FLUOstar Omega and a POLARstar Omega |
| Franka Ganske |
| BMG LABTECH 2008 |
| In this application note AlphaScreen® instrument settings and sensitivity as well as Z´value of a tyrosine kinase assay are presented. The measurements were done using Omega instruments. |
| Article |
| [179 - 2.580] |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Bonnett, S.A.F., Leah, R., and Maltby, E |
| BMG LABTECH 2008 |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| Article |
| [167 - 6.180] |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| Papadia S, Léveillé F and Hardingham GE |
| BMG LABTECH 2008 |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| Article |
| [152 - 1.140] |
| Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar |
| Ganske F, Dell EJ, Larson B |
| BMG LABTECH 2008 |
| Far-red competitive FP immunoassay for ATP depletion measurements |
| Article |
| [126 - 7.100] |
| PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays |
| Orban M, Angersbach S, Hoffman R, Buros M. |
| BMG LABTECH 2005 |
| Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique. |
| Application Note PDF |
| [151 - 4.250] |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Cali J, Dell EJ |
| Promega Corporation, BMG LABTECH, Germany |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| Article |
| [153 - 7.110] |
| Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode |
| Ganske F and Orban M. |
| BMG LABTECH, Germany, 2007 |
| Laser-based AlphaScreen® is demonstrated in this application note. PHERAstar instrument settings are given and a titration curve of biotinylated and phosphorylated polypeptide (biot-LCK-P) is presented. |
| Article |
| [141 - 2.450] |
| ADP HunterTM Assay for HTS of Kinase Inhibitors using the PHERAstar |
| Kauffman L, Hguyen U, Angersbach S, Orban M |
| BMG LABTECH 2006 |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| Article |
| [132 - 2.400] |
| Development of a post-reaction derivatisation and Fluorescence assay for 2-OG dependent dioxygenases |
| McNeill LA, Hewitson KS, Schonfeld CJ, Bethge L. |
| BMG LABTECH 2004 |
| NOVOstar was used both as incubator and reader for measuring the activity of dioxygenases |
| Article |
| [131 - 4.180] |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Paine A, Walker L. |
| BMG LABTECH 2004 |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| Article |
| [127 - 3.127] |
| A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar |
| Rossant CJ, Brown JP, Marshall ML. |
| BMG LABTECH 2004 |
| Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition |
| Article |
Second Messenger Signalling; cAMP
| [221 - 4.370] |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| K. Atze, B. Ortmann, S. Valentin, M. Kühn, S. Franke, N. Breuer, P. Stecha, B. Binkowski, L. de Bruin, A. Pitt |
| BMG LABTECH 2012 |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| Article |
| [209 - 3.200] |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex" Cell-based Assay |
| Jacquemart L, Dell EJ |
| BMG LABTECH 2010 |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection |
| Article |
| [126 - 7.100] |
| PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays |
| Orban M, Angersbach S, Hoffman R, Buros M. |
| BMG LABTECH 2005 |
| Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique. |
| Application Note PDF |
| [134 - 4.200] |
| New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar |
| Kauffman L, De La Llera S, Orban M |
| BMG LABTECH 2006 |
| Directly assess either Gi or Gs coupled GPCRs in cells in up to 1536-well format |
| Article |
Second messenger Signalling; Calcium
| [216 - 4.350] |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Bodo Ortmann, Silke Valentin, Kristin Atze, Nadine Breuer, Steffi Franke, Mathias Kühn and Leon de Bruin |
| BMG LABTECH Application Note 2011 |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| Article |
| [206 - 2.680] |
| Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader |
| Mark Koeppel, Invitrogen, Madison Wisconsin, USA; EJ Dell, BMG LABTECH, NC, USA |
| BMG LABTECH 2010 |
| Two fluorescent cell-based assays from Invitrogen performed on the PHERAstar FS with direct bottom reading and high resolution well scanning |
| Article |
| [157 - 2.500] |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| Liao J, Dell E.J. |
| ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007 |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| Article |
| [104 - 2.025] |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Heding A. |
| BMG LABTECH |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| Article |
| [107 - 2.013] |
| Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader |
| Hoefgen B, Kassack MU. |
| Calcium measurements of Oregon Green loaded HEK293 cells |
| Application Note PDF |
Second messenger Signalling; Inositol Phosphate
| [222 - 3.230] |
| HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway |
| Dell EJ, Tardieu JL, Degorce F |
| BMG LABTECH 2012 |
| Several low affinity drug hits were only found by the PMT-based PHERAstar FS microplate reader and not with a CCD-based instrument from a different vendor |
| Article |
| [209 - 3.200] |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex" Cell-based Assay |
| Jacquemart L, Dell EJ |
| BMG LABTECH 2010 |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection |
| Article |
| [142 - 1.030] |
| HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar |
| Kauffman L, De La Llera, Angersbach S, Orban M |
| BMG LABTECH 2006 |
| A fluorescence polarization based assay from DiscoveRx to measure inositol-3 phosphate levels |
| Article |
| [137 - 3.130] |
| HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers |
| Orban M, Degorce F, Tardieu JL |
| BMG LABTECH 2005 |
| Comprehensive overview of GPCRs in response to known agonists |
| Article |
| [127 - 3.127] |
| A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar |
| Rossant CJ, Brown JP, Marshall ML. |
| BMG LABTECH 2004 |
| Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition |
| Article |
Ion Channels (Electrophysiology)
| [175 - 2.560] |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| Heather Mortiboys and Oliver Bandmann |
| BMG LABTECH 2008 |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| Article |
| [173 - 1.150] |
| Performance of the PredictorTM hERG Fluorescence Polarization Assay Kit using the PHERAstar |
| Dell EJ |
| BMG LABTECH 2008 |
| Nonradioactive patch-clamp alternative to test drug candidates for blockage of the hERG channel |
| Article |
| [123 - 8.110] |
| Ion channel assay development using Invitrogen's FRET-based voltage sensor probes |
| Hoffman RL, Duff S, Qadir N. |
| Invitrogen Corporation, Madison, WI 53719, USA |
| POLARstar OPTIMA and NOVOstar plate readers are suitable platforms for development of VSP ion channel assays. |
| Article |
HTRF (Homogeneous Time-Resolved Fluorescence) Assays
| [193 - 3.190] |
| Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH |
| Bernd Laffert and Franka Ganske |
| BMG LABTECH |
| HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany. |
| Article |
| [177 - 3.170] |
| HTRF®IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus |
| Ganske F, Jacquemart L, Jean A, Lebreton ML, Tardieu JL |
| BMG LABTECH 2008 |
| An HTRF® assay to investigate all classes of compounds involved in the Gq activation pathway |
| Article |
| [126 - 7.100] |
| PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays |
| Orban M, Angersbach S, Hoffman R, Buros M. |
| BMG LABTECH 2005 |
| Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique. |
| Application Note PDF |
| [154 - 3.160] |
| Cisbio´s HTRF® cortisol assay performed on the PHERAstar |
| Ganske F, Tardieu JL and Degorce F. |
| BMG LABTECH, Germany and Cisbio International, France |
| Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined |
| Article |
| [137 - 3.130] |
| HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers |
| Orban M, Degorce F, Tardieu JL |
| BMG LABTECH 2005 |
| Comprehensive overview of GPCRs in response to known agonists |
| Article |
Solubility Measured with Nephelometry
| [174 - 5.120] |
| Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH's NEPHELOstar |
| U.-C. Hipler |
| BMG LABTECH 2008 |
| Laser nephelometry used for fungal growth determination in presence of antifungal agents |
| Article |
| [117 - 5.101] |
| A fully automated solubility screen in 384-well plate format using nephelometry |
| Green C, McKee S, Saunders K. |
| BMG LABTECH 2003 |
| Kinetic solubility determination of 24 compounds in 75 min |
| Article |
Single Nucleotide Polymorphisms (SNP Genotyping)
| [187 - 2.640] |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader |
| Hamilton A, Liang R, Whittle B |
| BMG LABTECH 2010 |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| Article |
| [160 - 8.130] |
| High Speed FRET based SNP Genotyping Measurement on the PHERAstar |
| Robinson P and Ganske F |
| BMG LABTECH 2007 |
| KASPar™ System from KBioScience optimally measured with dual emission optical modules |
| Article |
| [129 - 8.120] |
| Use of the PHERAstar for SNP genotyping chemistry development and high throughput production genotyping |
| Robinson P. |
| BMG LABTECH 2004 |
| SNP Genotyping using Fam, Hex and Rox as labels |
| Article |
Reporter Gene Assays
| [233 - 4.400] |
| Simultaneous dual-emission detection of luciferase reporter assays |
| Megan Dobbs, B.S., Douglas Hughes, Ph.D., Janaki Narahari, Ph.D., Jae Choi, Ph.D., Georgyi Los, Ph.D., Brian Webb, Ph.D. Thermo Fisher Scientific, Rockford, IL Eric Matthews, M.S., Carl Peters, Ph.D., BMG LABTECH, Cary, NC |
| BMG LABTECH 2012 |
| Dual-luciferase reporter assays measured with luminescence filters to separate luciferase signals. |
| Application Note PDF |
| Article |
| [221 - 4.370] |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| K. Atze, B. Ortmann, S. Valentin, M. Kühn, S. Franke, N. Breuer, P. Stecha, B. Binkowski, L. de Bruin, A. Pitt |
| BMG LABTECH 2012 |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| Article |
| [199 - 4.330] |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Eboigbodin KE and Robinson GK |
| BMG LABTECH 2009 |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| Article |
| [184 - 6.240] |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega |
| Mutch BE, Willetts K, Timms E, Foxwell BMJ |
| BMG LABTECH 2008 |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| Article |
| [172 - 4.300] |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| Gretton S, Harris M |
| BMG LABTECH 2008 |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| Article |
| [165 - 4.280] |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| Dell EJ |
| BMG LABTECH 2007 |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| Article |
Protein-Protein/Protein-DNA interactions
| [231 - 6.350] |
| Label-Free Kinetic Measurements Using SoPRano" Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader |
| Roskamp M, Meuvis J, Myszka D, Dell EJ |
| BMG LABTECH 2012 |
| Article |
| [230 - 6.340] |
| Label-Free SoPRano" Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader |
| Dell EJ, Roskamp M, Van de Velde F |
| BMG LABTECH 2012 |
| The label-free SoPRano" assay from Pharma Diagnostics were performed on the SPECTROstar Omega microplate reader |
| Article |
| [213 - 2.720] |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Miriam Uppill, Antony Sheehan and Ron Osmond |
| BMG LABTECH 2011 |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega |
| Article |
| [190 - 7.130] |
| Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus |
| Franka Ganske and Keith Ansell |
| BMG LABTECH 2009 |
| Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented. |
| Article |
| [180 - 1.160] |
| High-throughput protein-DNA affinity measurements using fluorescence anisotropy |
| Veprintsev DB |
| BMG LABTECH 2008 |
| The effect of modified nucleotides on the recognition of DNA by the tumour suppressor protein p53 was studied. |
| Article |
Microbial Growth / Cellular Analysis
Cell Growth Kinetics
| [155 - 6.150] |
| Growth of Neisseria meningitidis in the FLUOstar OPTIMA with Microprocessor Controlled Gas Vent |
| Cutter KL |
| BMG LABTECH Application Notes 2007 |
| This study highlights the advantage of an automated controlled gas system over a carbon dioxide incubator. |
| Article |
| [217 - 6.320] |
| Growth of Campylobacter using BMG LABTECH's FLUOstar Omega equipped with ACU |
| Haigh RD and Ketley JM |
| BMG LABTECH 2011 |
| Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU |
| Article |
| [207 - 6.300] |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2010 |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| Article |
| [199 - 4.330] |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Eboigbodin KE and Robinson GK |
| BMG LABTECH 2009 |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| Article |
| [191 - 6.270] |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure on the FLUOstar OPTIMA |
| S. Lucas Black, F. Bruce Ward and Rosalind J. Allen |
| BMG LABTECH 2009 |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| Article |
| [189 - 6.260] |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega |
| Gordon Ramage, Ryan Quinn and Steven Milligan |
| BMG LABTECH 2009 |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
| Article |
| [174 - 5.120] |
| Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH's NEPHELOstar |
| U.-C. Hipler |
| BMG LABTECH 2008 |
| Laser nephelometry used for fungal growth determination in presence of antifungal agents |
| Article |
| [140 - 6.130] |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| Webber MA, Piddock LJ |
| BMG LABTECH 2006 |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| Article |
| [125 - 5.106] |
| Monitoring of Microbial Growth Curves by Laser Nephelometry |
| Angersbach S, Brand S, Hipler B, Rückert C. |
| BMG LABTECH 2004 |
| Correlation between nephelometry and absorbance in microbial growth monitoring |
| Article |
Detection of Cell Contaminations and Mycoplasma
| [130 - 4.170] |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Cox A, Pitt A. |
| BMG LABTECH 2004 |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| Article |
| [114 - 5.005] |
| Nephelometric Test for Determination of Colostrum Quality |
| AgResearch, BMG LABTECH Australia |
| Quantification of IgG1 in milk and colostrum |
| Article |
Cell Viability Assays
| [228 - 6.360] |
| Biocolor's APOPercentage Apoptosis Assay(TM) on BMG LABTECH's SPECTROstar Nano plate reader |
| Morrow, J.C. and Steele, D. |
| BMG LABTECH 2012 |
| The APOPercentage apoptosis assay(TM) is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano. |
| Article |
| [218 - 4.360] |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| Andrew Knight, Chris Hughes and Matthew Tate. Gentronix Limited, Manchester, UK. |
| BMG LABTECH 2011 |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| Article |
| [214 - 2.730]a |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Emily Peak, Iain W. Chalmers and Karl F. Hoffmann |
| BMG LABTECH 2011 |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability |
| Article |
| [207 - 6.300] |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2010 |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| Article |
| [195 - 2.610] |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Markaki AE |
| BMG LABTECH 2009 |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| Article |
| [159 - 2.510] |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Chitolie MS & Toescu EC |
| BMG LABTECH 208 |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| Article |
| [171 - 4.290] |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Rebecca Foster and Kyla Grimshaw |
| BMG LABTECH 2008 |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay. |
| Article |
| [139 - 4.220] |
| Promega's Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar |
| Worzella T, Larson B |
| BMG LABTECH 2006 |
| Multiplexing reporter gene activity (EnduRen™ or ViviRen™) and cell viability (CellTiter-Glo®) in a single sample well |
| Article |
| [138 - 2.440] |
| Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar |
| Worzella T, Larson B |
| BMG LABTECH 2006 |
| Correlated measurement of an apoptosis marker and cell viability |
| Article |
Cell Cytotoxicity Assays
| [218 - 4.360] |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| Andrew Knight, Chris Hughes and Matthew Tate. Gentronix Limited, Manchester, UK. |
| BMG LABTECH 2011 |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| Article |
| [207 - 6.300] |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2010 |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| Article |
| [192 - 3.180] |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay performed on the PHERAstar |
| Patterson NM, Cunningham T, Jiang X, Dell EJ, Ganske F, Shapiro DJ |
| BMG LABTECH 2009 |
| Estrogen reduces cell death induced by NK92 natural killer cells |
| Article |
| [113 - 4.007] |
| The use of an ATP bioluminescence assay to quantify cell cytotoxicity |
| Hipler B. |
| BMG LABTECH |
| Classical ATP cell cytotoxicity assay based on firefly activity |
| Article |
GFP Expression
| [144 - 2.460] |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Hart CA , Clarke NW, Brown MD |
| Paterson Institute for Cancer Research, University of Manchester, Manchester, UK |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| Article |
| [119 - 1.101] |
| Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence |
| Knight AW |
| BMG LABTECH |
| The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay |
| Article |
Tight Junctions
| [146 - 2.470] |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| Balda MS and Matter K |
| BMG LABTECH 2007 |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| Article |
Macrophages
| [188 - 2.600] |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| Sanders, D.A. and Charnock-Jones, D.S. |
| BMG LABTECH 2009 |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| Article |
| [184 - 6.240] |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega |
| Mutch BE, Willetts K, Timms E, Foxwell BMJ |
| BMG LABTECH 2008 |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| Article |
| [156 - 2.490] |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Taylor A, Donelly L. |
| Thoracic Medicine, National Heart and Lung Institute |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| Article |
False Positives
| [124 - 1.020] |
| Identifcation of false positives in an FP Screen |
| Saxty B |
| BMG LABTECH 2004 |
| Identification of interfering compounds and non specific inhibitors |
| Article |
pH determination
| [219 - 3.220] |
| Assessment of Extracellular Acidification using a Fluorescence-Based Assay |
| Hynes J, Carey C, Kriwan S, Wark C |
| BMG LABTECH 2011 |
| Extracellular pH measurement in TRF mode using Luxcel chemistry |
| Article |
Food Chemistry
Antioxidant Determination (ORAC and ABEL®)
| [197 - 2.630] |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| Franka Ganske und Marjan Orban |
| BMG LABTECH 2009 |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| Article |
| [148 - 2.480] |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| Ganske F, Dell EJ |
| BMG LABTECH |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| Article |
| [108 - 4.009] |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Hipler B, Knight J. |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk |
| Article |
Reactive Oxygen species (ROS) and (Chemiluminescence)
| [112 - 4.103] |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Lateef S, Ingram PR, Spickett CM. |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| Article |
| [111 - 4.006] |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Hipler B, Hipler UC. |
| Very low ROS concentrations in Candida species detected |
| Article |
| [106 - 4.002] |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| Hipler UC, Schröter C, Wollina U, Hipler B. |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| Article |
| [105 - 2.008] |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| Hipler UC, Wollina U, Denning D, Hipler B. |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2) |
| Application Note PDF |
Oxygen Sensing
| [223 - 3.340] |
| Real-time Monitoring of Intracellular Oxygen Using MitoXpress®-Intra |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2012 |
| Easily assess transient changes in metabolic activity using the FLUOstar Omega equipped with ACU in conjunction with a membrane-permeable, ultra-sensitive reagent from Luxcel Biosciences |
| Article |
| [207 - 6.300] |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2010 |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| Article |
Food and Beverages Quality Testing
| [226 - 6.350] |
| A New Way to Test the Free Amino Nitrogen Content in Alcoholic Beverages with the SPECTROstar Nano |
| Spedding G, Harrison NR, Ganske F, Dell EJ |
| BMG LABTECH 2012 |
| Ninhydrin-based absorbance assay detects free amino nitrogen in beer samples using the SPECTROstar Nano in cuvette and microplate mode |
| Article |
| [223 - 3.340] |
| Real-time Monitoring of Intracellular Oxygen Using MitoXpress®-Intra |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2012 |
| Easily assess transient changes in metabolic activity using the FLUOstar Omega equipped with ACU in conjunction with a membrane-permeable, ultra-sensitive reagent from Luxcel Biosciences |
| Article |
| [219 - 3.220] |
| Assessment of Extracellular Acidification using a Fluorescence-Based Assay |
| Hynes J, Carey C, Kriwan S, Wark C |
| BMG LABTECH 2011 |
| Extracellular pH measurement in TRF mode using Luxcel chemistry |
| Article |
| [207 - 6.300] |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| Hynes J, Carey C, Wark C |
| BMG LABTECH 2010 |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| Article |
Additional Assays
BRET (Bioluminescence resonance energy transfer) assays
| [120 - 9.110] |
| Simultaneous dual emission detection for fast kinetic BRET assays |
| Orban M. |
| BMG LABTECH 2003 |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| Article |
| [118 - 9.100] |
| BRET assays performed on the FLUOstar OPTIMA |
| Orban M. |
| BMG LABTECH 2002 |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| Application Note PDF |
FRET (Fluorescence Resonance Energy Transfer) assays
| [194 - 8.150] |
| FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction |
| Hutsell SQ, Kimple AJ, Siderovski DP |
| BMG LABTECH 2009 |
| CFP-YFP FRET based assay utilized to screen for potential inhibitors |
| Article |
| [163 - 8.140] |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Martin SF |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| Article |
| [122 - 8.100] |
| Use of CyDye Fluors for Improved FRET Protease Assays on BMG POLARstar Fluorescence Microplate Reader |
| George J, Norey C. |
| GE Healthcare |
| CyDye™ labeled peptides are used as substrates in protease assays |
| Article |
| Link |
Migration Assay
| [204 - 2.670] |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Iman van den Bout |
| BMG LABTECH 2009 |
| Real-time monitoring of cell migration on different ECM components |
| Article |
| [144 - 2.460] |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Hart CA , Clarke NW, Brown MD |
| Paterson Institute for Cancer Research, University of Manchester, Manchester, UK |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| Article |
ADME (Absorption, Distribution, Metabolism and Elimination)
| [151 - 4.250] |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Cali J, Dell EJ |
| Promega Corporation, BMG LABTECH, Germany |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| Article |
Hormone Signalling
| [154 - 3.160] |
| Cisbio´s HTRF® cortisol assay performed on the PHERAstar |
| Ganske F, Tardieu JL and Degorce F. |
| BMG LABTECH, Germany and Cisbio International, France |
| Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined |
| Article |
Other
| [178 - 6.230] |
| Reaction optimization by parallel kinetic studies with the FLUOstar Omega |
| Redman JE |
| BMG LABTECH 2008 |
| Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel. |
| Article |
| [145 - 6.140] |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| Wiles M. |
| Plasso Technology Ltd., Sheffield, UK |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| Article |
| [147 - 4.240] |
| LUMIstar in the production and quality control for ROTAS on-site test reagents |
| Dix A |
| Cybersense Biosystems Ltd, Oxon, UK |
| BMG LABTECH microplate reader used in the quality control of reagent manufacturing |
| Article |
| [136 - 4.210] |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Perfect E, Hickey P. |
| BMG LABTECH 2004 |
| Measurement of certified calibration reference materials in comparison to biological controls |
| Article |
Membranes
| [208 - 2.690] |
| A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition |
| Melchior DL, Sharom FJ, Dell EJ |
| BMG LABTECH 2010 |
| The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter |
| Article |
| [205 - 1.190] |
| Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega |
| Kushnareva Y, Dell EJ |
| BMG LABTECH 2009 |
| DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes |
| Article |
Aggregation
| [215 - 6.310] |
| Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader |
| Vaiyapuri S and Gibbins JM |
| BMG LABTECH 2011 |
| Platelet aggregation induced by addition of agonist measured on the NOVOstar in absorbance mode. |
| Article |
| [212 - 2.710] |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Alison Turner UCB |
| BMG LABTECH 2011 |
| Thioflavin T aggregation assay to determine antibody stability |
| Article |
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