Application Notes sorted by Microplate Reader
BMG LABTECH has published a wide variety of application notes that were prepared either in-house or in collaboration with users from academia or pharma. Click on the microplate reader of interest below to have a look at all compatible application notes.
PHERAstar FS
| AN 231 |
| Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader |
| Protein-Protein Binding Kinetics Using the label-free SoPRano™ Assay from Pharma Diagnostics, a Spectrometer-based Microplate Reader, and MARS Data Analysis software |
| AN 230 |
| Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader |
| The label-free SoPRano™ assay from Pharma Diagnostics were performed on the SPECTROstar Omega microplate reader |
| AN 229 |
| Nano High-Throughput-Screening (nHTS) Platform - Miniaturization of Cell-based GPCR and Kinase Assays |
PHERAstar FS measures chemiluminescence-based screening assays from Discoverx in 1536 and 3456-well plate format |
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 227 |
| Quantifying Fluorescent Ligand Binding to GPCRs in Live Cells using the PHERAstar FS - a new format for HTS |
| Fluorescent dopamine and adenosine ligand binding assays in living cells were performed on the PHERAstar FS using the focal height adjustment and bottom reading mode. |
| AN 225 |
| Methyltransferase, Acetyltransferase, Kinases, and GTPases Can All Be Measured with Transcreener® Assays |
| All four Transcreener FP assays for nucleotide detection show excellent Z´values and a large signal window on the PHERAstar |
| AN 222 |
| HTS Instrument Discovers Low Affinity Inhibitors of the Inositol Phosphate (IP) Signaling Pathway |
| Several low affinity drug hits were only found by the PMT-based PHERAstar FS microplate reader and not with a CCD-based instrument from a different vendor |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 218 |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 211 |
| Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus |
Nucleic acid quantification on the PHERAstar Plus microplate reader in fluorescence mode in 384-well format. |
| AN 210 |
| An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on the POLARstar Omega |
| POLARstar Omega is used to detect ERK phosphorylation using an AlphaScreen® SureFire® proximity assay. |
| AN 209 |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex Cell-based Assay |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection. |
| AN 208 |
| A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition |
| The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter. |
| AN 206 |
| Cellular Dopamine and Intracellular Calcium Signaling Using the PHERAstar FS |
| Two fluorescent cell-based assays from Invitrogen performed on the PHERAstar FS with direct bottom reading and high resolution well scanning. |
| AN 205 |
| Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega |
| DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes. |
| AN 204 |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Real-time monitoring of cell migration on different ECM components |
| AN 203 |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 201 |
| The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers |
| The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay. |
| AN 199 |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| AN 197 |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| AN 196 |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Alternative, non-radiological method to assess heterotrimeric G protein activation. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 194 |
| FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction |
| CFP-YFP FRET based assay utilized to screen for potential inhibitors. |
| AN 193 |
| Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH |
| HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany. |
| AN 192 |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay on the PHERAstar |
| Estrogen reduces cell death induced by NK92 natural killer cells. |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 190 |
| Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus |
| Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
AN 188 |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| AN 187 |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| AN 184 |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| AN 182 |
| New Transcreener® ADP2 FP Assay performed on the PHERAstar Plus Microplate Reader |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| AN 180 |
| High-throughput protein-DNA affinity measurements using fluorescence anisotropy |
| The effect of modified nucleotides on the recognition of DNA by the tumour suppressor protein p53 was studied. |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 178 |
| Reaction optimization by parallel kinetic studies with the FLUOstar Omega |
| Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel. |
| AN 177 |
| HTRF® IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus |
| An HTRF® assay to investigate all classes of compounds involved in the Gq activation pathway. |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 175 |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| AN 173 |
| Performance of the Predictor™ hERG Fluorescence Polarization Assay Kit using the PHERAstar |
| Nonradioactive patch-clamp alternative to test drug candidates for blockage of the hERG channel |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 164 |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| AN 163 |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 160 |
| High Speed FRET based SNP Genotyping Measurement on the PHERAstar |
| KASPar™ System from KBioScience optimally measured with dual emission optical modules |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 157 |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| AN 156 |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| AN 154 |
| Cisbio´s HTRF® cortisol assay performed on the PHERAstar |
| Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined |
| AN 153 |
| Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode |
| Laser-based AlphaScreen® is demonstrated in this application note. PHERAstar instrument settings are given and a titration curve of biotinylated and phosphorylated polypeptide (biot-LCK-P) is presented. |
| AN 152 |
| Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar |
| Far-red competitive FP immunoassay for ATP depletion measurements. |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 148 |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| AN 147 |
| LUMIstar in the production and quality control for ROTAS on-site test reagents |
| BMG LABTECH microplate reader used in the quality control of reagent manufacturing |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 144 |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 142 |
| HitHunter™ IP3 Assay for GCPR Screening using the PHERAstar |
| A fluorescence polarization based assay from DiscoveRx to measure inositol-3 phosphate levels |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 139 |
| Promega's Multiplexed Luciferase Reporter and Cell Viability Assays performed on PHERAstar |
| Multiplexing reporter gene activity (EnduRen™ or ViviRen™) and cell viability (CellTiter-Glo®) in a single sample well. |
| AN 138 |
| Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar |
| Correlated measurement of an apoptosis marker and cell viability |
| AN 137 |
| HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers |
| Comprehensive overview of GPCRs in response to known agonists |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 134 |
| New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar |
| Directly assess either Gi or Gs coupled GPCRs in cells in up to 1536-well format |
| AN 132 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 129 |
| Use of the PHERAstar for SNP genotyping chemistry development and high throughput production genotyping |
| SNP Genotyping using Fam, Hex and Rox as labels |
| AN 128 |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
AN 127 |
| A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar |
| Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition |
| AN 126 |
| PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays |
| Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique. |
| AN 124 |
| Identifcation of false positives in an FP Screen |
| Identification of interfering compounds and non specific inhibitors |
| AN 123 |
| Ion channel assay development using Invitrogen's FRET-based voltage sensor probes |
| POLARstar OPTIMA and NOVOstar plate readers are suitable platforms for development of VSP ion channel assays. |
| AN 122 |
| Use of CyDye Fluors for Improved FRET Protease Assays on POLARstar |
| CyDye™ labeled peptides are used as substrates in protease assays |
| AN 120 |
| Simultaneous dual emission detection for fast kinetic BRET assays |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 119 |
| Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence |
| The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay |
| AN 118 |
| BRET assays performed on the FLUOstar OPTIMA |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 104 |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
PHERAstar Plus
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 225 |
| Methyltransferase, Acetyltransferase, Kinases, and GTPases Can All Be Measured with Transcreener® Assays |
| All four Transcreener FP assays for nucleotide detection show excellent Z´values and a large signal window on the PHERAstar |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 211 |
| Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus |
Nucleic acid quantification on the PHERAstar Plus microplate reader in fluorescence mode in 384-well format. |
| AN 210 |
| An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on the POLARstar Omega |
| POLARstar Omega is used to detect ERK phosphorylation using an AlphaScreen® SureFire® proximity assay. |
| AN 209 |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex Cell-based Assay |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection. |
| AN 205 |
| Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega |
| DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 201 |
| The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers |
| The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 194 |
| FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction |
| CFP-YFP FRET based assay utilized to screen for potential inhibitors. |
| AN 193 |
| Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH |
| HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany. |
| AN 192 |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay on the PHERAstar |
| Estrogen reduces cell death induced by NK92 natural killer cells. |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 190 |
| Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus |
| Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
| AN 182 |
| New Transcreener® ADP2 FP Assay performed on the PHERAstar Plus Microplate Reader |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| AN 180 |
| High-throughput protein-DNA affinity measurements using fluorescence anisotropy |
| The effect of modified nucleotides on the recognition of DNA by the tumour suppressor protein p53 was studied. |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 177 |
| HTRF® IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus |
| An HTRF® assay to investigate all classes of compounds involved in the Gq activation pathway. |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 173 |
| Performance of the Predictor™ hERG Fluorescence Polarization Assay Kit using the PHERAstar |
| Nonradioactive patch-clamp alternative to test drug candidates for blockage of the hERG channel |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 160 |
| High Speed FRET based SNP Genotyping Measurement on the PHERAstar |
| KASPar™ System from KBioScience optimally measured with dual emission optical modules |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 154 |
| Cisbio´s HTRF® cortisol assay performed on the PHERAstar |
| Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined |
| AN 153 |
| Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode |
| Laser-based AlphaScreen® is demonstrated in this application note. PHERAstar instrument settings are given and a titration curve of biotinylated and phosphorylated polypeptide (biot-LCK-P) is presented. |
| AN 152 |
| Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar |
| Far-red competitive FP immunoassay for ATP depletion measurements. |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 142 |
| HitHunter™ IP3 Assay for GCPR Screening using the PHERAstar |
| A fluorescence polarization based assay from DiscoveRx to measure inositol-3 phosphate levels |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 139 |
| Promega's Multiplexed Luciferase Reporter and Cell Viability Assays performed on PHERAstar |
| Multiplexing reporter gene activity (EnduRen™ or ViviRen™) and cell viability (CellTiter-Glo®) in a single sample well. |
| AN 138 |
| Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar |
| Correlated measurement of an apoptosis marker and cell viability |
| AN 137 |
| HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers |
| Comprehensive overview of GPCRs in response to known agonists |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 134 |
| New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar |
| Directly assess either Gi or Gs coupled GPCRs in cells in up to 1536-well format |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 129 |
| Use of the PHERAstar for SNP genotyping chemistry development and high throughput production genotyping |
| SNP Genotyping using Fam, Hex and Rox as labels |
AN 127 |
| A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar |
| Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition |
| AN 126 |
| PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays |
| Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique. |
| AN 124 |
| Identifcation of false positives in an FP Screen |
| Identification of interfering compounds and non specific inhibitors |
| AN 122 |
| Use of CyDye Fluors for Improved FRET Protease Assays on POLARstar |
| CyDye™ labeled peptides are used as substrates in protease assays |
| AN 119 |
| Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence |
| The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
| AN 231 |
| Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader |
| Protein-Protein Binding Kinetics Using the label-free SoPRano™ Assay from Pharma Diagnostics, a Spectrometer-based Microplate Reader, and MARS Data Analysis software |
| AN 230 |
| Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader |
| The label-free SoPRano™ assay from Pharma Diagnostics were performed on the SPECTROstar Omega microplate reader |
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 224 |
| Assessing pancreatic trypsin activity using the POLARstar Omega Microplate Reader |
| Fluorimetric kinetic trypsin activity assay performed on a POLARstar Omega as a measure for acute pancreatitis. |
| AN 223 |
| Real-time Monitoring of Intracellular Oxygen Using MitoXpress®-Intra |
| Easily assess transient changes in metabolic activity using the FLUOstar Omega equipped with ACU in conjunction with a membrane-permeable, ultra-sensitive reagent from Luxcel Biosciences. |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 219 |
| Assessment of Extracellular Acidification using a Fluorescence-Based Assay |
Extracellular pH measurement in TRF mode using Luxcel chemistry. |
| AN 218 |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| AN 217 |
| Growth of Campylobacter using BMG LABTECH's FLUOstar Omega equipped with ACU |
| Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 211 |
| Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus |
Nucleic acid quantification on the PHERAstar Plus microplate reader in fluorescence mode in 384-well format. |
| AN 210 |
| An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on the POLARstar Omega |
| POLARstar Omega is used to detect ERK phosphorylation using an AlphaScreen® SureFire® proximity assay. |
| AN 209 |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex Cell-based Assay |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection. |
| AN 208 |
| A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition |
| The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter. |
| AN 207 |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| AN 205 |
| Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega |
| DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes. |
| AN 204 |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Real-time monitoring of cell migration on different ECM components |
| AN 203 |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 201 |
| The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers |
| The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay. |
| AN 199 |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| AN 197 |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| AN 196 |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Alternative, non-radiological method to assess heterotrimeric G protein activation. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 194 |
| FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction |
| CFP-YFP FRET based assay utilized to screen for potential inhibitors. |
| AN 193 |
| Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH |
| HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany. |
| AN 192 |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay on the PHERAstar |
| Estrogen reduces cell death induced by NK92 natural killer cells. |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 190 |
| Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus |
| Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
AN 188 |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| AN 187 |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| AN 186 |
| AlphaScreen® Phosphotyrosine Assay Performed on a FLUOstar Omega and a POLARstar Omega |
| In this application note AlphaScreen® instrument settings and sensitivity as well as Z´value of a tyrosine kinase assay are presented. The measurements were done using Omega instruments. |
| AN 184 |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| AN 183 |
| New Transcreener® ADP2 FP Assay performed on BMG LABTECH's POLARstar Omega |
| The Transcreener® FP technology from BellBrookLabs allows to monitor any enzymatic reaction that produces ADP |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 178 |
| Reaction optimization by parallel kinetic studies with the FLUOstar Omega |
| Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel. |
| AN 177 |
| HTRF® IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus |
| An HTRF® assay to investigate all classes of compounds involved in the Gq activation pathway. |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 175 |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 164 |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| AN 163 |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 157 |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| AN 156 |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| AN 155 |
| Growth of Neisseria meningitidis in the FLUOstar OPTIMA with Microprocessor Controlled Gas Vent |
| This study highlights the advantage of an automated controlled gas system over a carbon dioxide incubator. |
| AN 154 |
| Cisbio´s HTRF® cortisol assay performed on the PHERAstar |
| Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 148 |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 144 |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 137 |
| HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers |
| Comprehensive overview of GPCRs in response to known agonists |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 132 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 128 |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
AN 127 |
| A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar |
| Measuring phosphatidyl inositol-3 kinase (PI-3 kinase) activity and inhibition |
| AN 126 |
| PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays |
| Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique. |
| AN 124 |
| Identifcation of false positives in an FP Screen |
| Identification of interfering compounds and non specific inhibitors |
| AN 123 |
| Ion channel assay development using Invitrogen's FRET-based voltage sensor probes |
| POLARstar OPTIMA and NOVOstar plate readers are suitable platforms for development of VSP ion channel assays. |
| AN 122 |
| Use of CyDye Fluors for Improved FRET Protease Assays on POLARstar |
| CyDye™ labeled peptides are used as substrates in protease assays |
| AN 120 |
| Simultaneous dual emission detection for fast kinetic BRET assays |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 119 |
| Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence |
| The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 104 |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
| AN 232 |
| Real-Time Quaking Induced Conversion Assay for Prion Seeding |
The Omega microplate readers were used to perform an assay for the determination of prion seeding while shaking for many days at high speed |
| AN 231 |
| Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader |
| Protein-Protein Binding Kinetics Using the label-free SoPRano™ Assay from Pharma Diagnostics, a Spectrometer-based Microplate Reader, and MARS Data Analysis software |
| AN 230 |
| Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader |
| The label-free SoPRano™ assay from Pharma Diagnostics were performed on the SPECTROstar Omega microplate reader |
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 224 |
| Assessing pancreatic trypsin activity using the POLARstar Omega Microplate Reader |
| Fluorimetric kinetic trypsin activity assay performed on a POLARstar Omega as a measure for acute pancreatitis. |
| AN 223 |
| Real-time Monitoring of Intracellular Oxygen Using MitoXpress®-Intra |
| Easily assess transient changes in metabolic activity using the FLUOstar Omega equipped with ACU in conjunction with a membrane-permeable, ultra-sensitive reagent from Luxcel Biosciences. |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 219 |
| Assessment of Extracellular Acidification using a Fluorescence-Based Assay |
Extracellular pH measurement in TRF mode using Luxcel chemistry. |
| AN 218 |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| AN 217 |
| Growth of Campylobacter using BMG LABTECH's FLUOstar Omega equipped with ACU |
| Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 211 |
| Measure femtogram quantities of dsDNA on the BMG LABTECH PHERAstar Plus |
Nucleic acid quantification on the PHERAstar Plus microplate reader in fluorescence mode in 384-well format. |
| AN 210 |
| An AlphaScreen® SureFire® Phospho-ERK1/2 assay performed on the POLARstar Omega |
| POLARstar Omega is used to detect ERK phosphorylation using an AlphaScreen® SureFire® proximity assay. |
| AN 209 |
| GPCR Activation is Measured with Cisbio's cAMP and IP-One HTRF® HTplex Cell-based Assay |
| Simultaneously measure Gai/s and Gaq signaling in the same assay using the PHERAstar and SDE detection. |
| AN 208 |
| A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition |
| The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter. |
| AN 207 |
| Assessing Microbial Metabolism Using A Simple "Mix & Measure" Oxygen Consumption Assay |
| MitoXpress from Luxcel Biosciences is a water-soluble oxygen-sensitive phosphorescent probe that facilitates microplate based analysis of microbial oxygen consumption. The microbial metabolism implications of treatments such as drug exposure, genetic manipulation or altered cultured conditions can be easily accessed using BMG LABTECH´s FLUOstar Omega microplate reader to read both growth and oxygen consumption in parallel. |
| AN 204 |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Real-time monitoring of cell migration on different ECM components |
| AN 203 |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 201 |
| The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers |
| The Transcreener® technology from BellBrookLabs utilizes a one-step competitive red fluorescence immunoassay. |
| AN 199 |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| AN 197 |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| AN 196 |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Alternative, non-radiological method to assess heterotrimeric G protein activation. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 193 |
| Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH |
| HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany. |
| AN 192 |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay on the PHERAstar |
| Estrogen reduces cell death induced by NK92 natural killer cells. |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 190 |
| Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus |
| Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
AN 188 |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| AN 187 |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| AN 186 |
| AlphaScreen® Phosphotyrosine Assay Performed on a FLUOstar Omega and a POLARstar Omega |
| In this application note AlphaScreen® instrument settings and sensitivity as well as Z´value of a tyrosine kinase assay are presented. The measurements were done using Omega instruments. |
| AN 184 |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 178 |
| Reaction optimization by parallel kinetic studies with the FLUOstar Omega |
| Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel. |
| AN 177 |
| HTRF® IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus |
| An HTRF® assay to investigate all classes of compounds involved in the Gq activation pathway. |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 175 |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 164 |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| AN 163 |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 157 |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| AN 156 |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| AN 155 |
| Growth of Neisseria meningitidis in the FLUOstar OPTIMA with Microprocessor Controlled Gas Vent |
| This study highlights the advantage of an automated controlled gas system over a carbon dioxide incubator. |
| AN 154 |
| Cisbio´s HTRF® cortisol assay performed on the PHERAstar |
| Activity of key metabolic enzyme measured and EC50 values for cortisol and IC50 values for common inhibitors determined |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 148 |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 144 |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 137 |
| HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers |
| Comprehensive overview of GPCRs in response to known agonists |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 132 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 128 |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
| AN 118 |
| BRET assays performed on the FLUOstar OPTIMA |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 104 |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 217 |
| Growth of Campylobacter using BMG LABTECH's FLUOstar Omega equipped with ACU |
| Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 147 |
| LUMIstar in the production and quality control for ROTAS on-site test reagents |
| BMG LABTECH microplate reader used in the quality control of reagent manufacturing |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 120 |
| Simultaneous dual emission detection for fast kinetic BRET assays |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 118 |
| BRET assays performed on the FLUOstar OPTIMA |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
SPECTROstar Omega
| AN 231 |
| Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader |
| Protein-Protein Binding Kinetics Using the label-free SoPRano™ Assay from Pharma Diagnostics, a Spectrometer-based Microplate Reader, and MARS Data Analysis software |
| AN 230 |
| Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader |
| The label-free SoPRano™ assay from Pharma Diagnostics were performed on the SPECTROstar Omega microplate reader |
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
| AN 178 |
| Reaction optimization by parallel kinetic studies with the FLUOstar Omega |
| Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel. |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 155 |
| Growth of Neisseria meningitidis in the FLUOstar OPTIMA with Microprocessor Controlled Gas Vent |
| This study highlights the advantage of an automated controlled gas system over a carbon dioxide incubator. |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
POLARstar OPTIMA
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 218 |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 215 |
| Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader |
| Platelet aggregation induced by addition of agonist measured on the NOVOstar in absorbance mode. |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 208 |
| A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition |
| The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter. |
| AN 205 |
| Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega |
| DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes. |
| AN 204 |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Real-time monitoring of cell migration on different ECM components |
| AN 203 |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 199 |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| AN 197 |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| AN 196 |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Alternative, non-radiological method to assess heterotrimeric G protein activation. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 194 |
| FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction |
| CFP-YFP FRET based assay utilized to screen for potential inhibitors. |
| AN 192 |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay on the PHERAstar |
| Estrogen reduces cell death induced by NK92 natural killer cells. |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
AN 188 |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| AN 187 |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| AN 185 |
| Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA |
| Esterase activity was determined using the p-nitrophenyl acetate (pNPA) assay. Km and Vmax were calculated using different calculation methods (e.g. Michaelis-Menten and Lineweaver-Burk). |
| AN 184 |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 175 |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 164 |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| AN 163 |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 157 |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| AN 156 |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 148 |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 144 |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 132 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 128 |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
| AN 124 |
| Identifcation of false positives in an FP Screen |
| Identification of interfering compounds and non specific inhibitors |
| AN 123 |
| Ion channel assay development using Invitrogen's FRET-based voltage sensor probes |
| POLARstar OPTIMA and NOVOstar plate readers are suitable platforms for development of VSP ion channel assays. |
| AN 122 |
| Use of CyDye Fluors for Improved FRET Protease Assays on POLARstar |
| CyDye™ labeled peptides are used as substrates in protease assays |
| AN 120 |
| Simultaneous dual emission detection for fast kinetic BRET assays |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 119 |
| Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence |
| The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 104 |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 224 |
| Assessing pancreatic trypsin activity using the POLARstar Omega Microplate Reader |
| Fluorimetric kinetic trypsin activity assay performed on a POLARstar Omega as a measure for acute pancreatitis. |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 218 |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 215 |
| Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader |
| Platelet aggregation induced by addition of agonist measured on the NOVOstar in absorbance mode. |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 208 |
| A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition |
| The Fluorosome®-trans-pgp fluorescence intensity assay specifically measures the inhibition of Pgp multidrug transporter. |
| AN 204 |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Real-time monitoring of cell migration on different ECM components |
| AN 203 |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 199 |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| AN 197 |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| AN 196 |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Alternative, non-radiological method to assess heterotrimeric G protein activation. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 192 |
| A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay on the PHERAstar |
| Estrogen reduces cell death induced by NK92 natural killer cells. |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
AN 188 |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| AN 187 |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| AN 185 |
| Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA |
| Esterase activity was determined using the p-nitrophenyl acetate (pNPA) assay. Km and Vmax were calculated using different calculation methods (e.g. Michaelis-Menten and Lineweaver-Burk). |
| AN 184 |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 175 |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 164 |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| AN 163 |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 157 |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| AN 156 |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 148 |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 144 |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 132 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 128 |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
| AN 118 |
| BRET assays performed on the FLUOstar OPTIMA |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 104 |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 147 |
| LUMIstar in the production and quality control for ROTAS on-site test reagents |
| BMG LABTECH microplate reader used in the quality control of reagent manufacturing |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 120 |
| Simultaneous dual emission detection for fast kinetic BRET assays |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 118 |
| BRET assays performed on the FLUOstar OPTIMA |
| BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
SPECTROstar Nano| TAN 001 |
| DNA measurements in low volume samples, microplates and cuvettes on the SPECTROstar Nano |
| Three different ways to measure DNA samples in the SPECTROstar Nano are in detail explained and advantages of each option as well as sensitivity data are presented. |
| AN 231 |
| Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader |
| Protein-Protein Binding Kinetics Using the label-free SoPRano™ Assay from Pharma Diagnostics, a Spectrometer-based Microplate Reader, and MARS Data Analysis software |
| AN 230 |
| Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader |
| The label-free SoPRano™ assay from Pharma Diagnostics were performed on the SPECTROstar Omega microplate reader |
| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 226 |
| A New Way to Test the Free Amino Nitrogen Content in Alcoholic Beverages with the SPECTROstar Nano |
| Ninhydrin-based absorbance assay detects free amino nitrogen in beer samples using the SPECTROstar Nano in cuvette and microplate mode |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
| AN 178 |
| Reaction optimization by parallel kinetic studies with the FLUOstar Omega |
| Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel. |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
NOVOstar| AN 228 |
| Biocolor's APOPercentage Apoptosis Assay™ on the SPECTROstar Nano plate reader |
| The APOPercentage apoptosis assay™ is a simple colorimetric method to detect the percentage of apoptosis in live cells using the SPECTROstar Nano |
| AN 224 |
| Assessing pancreatic trypsin activity using the POLARstar Omega Microplate Reader |
| Fluorimetric kinetic trypsin activity assay performed on a POLARstar Omega as a measure for acute pancreatitis. |
| AN 221 |
| Monitoring Intracellular cAMP with hMSC cAMP biosensor using the LUMIstar Omega |
| Lonza´s Primary cells, Promega´s GloSensor chemistry and the LUMIstar Omega from BMG LABTECH were combined to investigate GPCR signaling pathways triggering intracellular cAMP production. |
| AN 220 |
| A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1) |
| Fluorescent HDAC activity and inhibitor assays were performed on a POLARstar OPTIMA microplate reader. |
| AN 218 |
| BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay |
| A human-derived, p53-competent TK6 cell line is used to host a luminescence-based reporter system to access genotoxic stress. |
| AN 216 |
| Monitoring Intracellular Ca2+ Fluxes with CloneticsTM primary sensors using the LUMIstar Omega |
| Calcium flux monitoring in primary cells (HUVECs) using flash luminescence measurements |
| AN 215 |
| Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader |
| Platelet aggregation induced by addition of agonist measured on the NOVOstar in absorbance mode. |
| AN 214 |
| Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability |
| Dual chromatic bottom reading fluorescence assay to determine schistosoma viability. |
| AN 213 |
| The ELISAONE" assay performed on the POLARstar Omega from BMG LABTECH |
| Fluorescent based ELISA with only one wash step measured on the POLARstar Omega. |
| AN 212 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 205 |
| Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega |
| DHP used as membrane-bound fluorophore in both liposomes and mitochondrial membranes. |
| AN 204 |
| Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega |
| Real-time monitoring of cell migration on different ECM components |
| AN 203 |
| Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase |
| Michaelis Menten kinetics (Km and Vmax) determined for a library of 160 elastase inhibitors. |
| AN 202 |
| Promega's ADP-Glo™ Kinase Assay performed on the PHERAstar FS and POLARstar Omega |
| A homogeneous luminescent assay from Promega to detect ADP. |
| AN 199 |
| Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA |
| Effects on quorum sensing were investigated by measuring bioluminescence and bacterial growth. |
| AN 197 |
| ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader |
| The antioxidant capacity of fruit juices is determined in 96-well and 384-well format |
| AN 196 |
| Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation |
| Alternative, non-radiological method to assess heterotrimeric G protein activation. |
| AN 195 |
| alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA |
| Metabolic active cells and cell proliferation on different substrates were measured using a fluorescence-based assay |
| AN 191 |
| High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure |
| Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity. |
| AN 189 |
| High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics |
| The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic. |
AN 188 |
| A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA |
| A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated. |
| AN 187 |
| Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA |
| Detection of SNPs (single nucleotide polymorphisms) using a fluorescent kit from Millipore |
| AN 184 |
| Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages |
| The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR). |
| AN 179 |
| Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA |
| Methylumbelliferone (MUF) and derivatives were used as substrates for peat enzymes |
| AN 176 |
| Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA |
| The RPA technology is characterized by a simple sample preparation and a constant temperature operation in a homogeneous format |
| AN 175 |
| Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader |
| The membrane potential was measured in fibroblast cells in 96-well and 384-well format |
| AN 172 |
| Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon |
| DLR™ assay utilized to monitor early stage replication events on certified FLUOstar OPTIMA |
| AN 171 |
| Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega |
| Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay |
| AN 170 |
| Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer |
| UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm. |
| AN 169 |
| Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation |
| Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented. |
| AN 168 |
| Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader |
| DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented. |
| AN 167 |
| Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA |
| The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm. |
| AN 166 |
| Molecular Probes® NanoOrange® Assay Performed on the FLUOstar OPTIMA |
| Protein determination using a fluorescent assay from Invitrogen |
| AN 165 |
| Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers |
| DLR validation process for LUMIstar, FLUOstar and POLARstar Omega equipped with two onboard reagent injectors |
| AN 164 |
| Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation |
| The dye bis-ANS were used to detect amyloid formation in a script-supported kinetic experiment. |
| AN 163 |
| SUMO FRET-based assays performed on BMG LABTECH's NOVOstar |
| Screening and analyzing of small ubiquitin-like modifiers on the NOVOstar |
| AN 162 |
| Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence |
| Change in intrinsic tryptophan fluorescence is used to measure the binding of an antibody to its antigen |
| AN 159 |
| High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA |
| Cell viability of cerebellar granule cells were measured utilizing propidium iodide |
| AN 158 |
| Bradford Assay Performed on the FLUOstar Omega with new Evaluation Software |
| The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window. |
| AN 157 |
| Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest |
| NOVOstar´s unique pipetting system allows for instantaneous detection after addition of agonist |
| AN 156 |
| Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA |
| Fluorescently labelled polystyrene beads were used as targets for phagocytosis studies |
| AN 151 |
| Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA |
| Rapid and reliable detection of cytochrome P450 activity with the help of firefly luciferase |
| AN 148 |
| ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity |
| A standard assay to determine antioxidant capacity using Trolox® as reference substance |
| AN 146 |
| Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans |
| A tight junction permeability assay that analyzes tight junction integrity was measured on the FLUOstar OPTIMA |
| AN 145 |
| Plasso EpranEx™ ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader |
| A detection system to identify and characterise heparin binding proteins is introduced. |
| AN 144 |
| Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA |
| Cell migration was followed in real-time analysis using the BD Fluoro-Blok" system |
| AN 143 |
| Study of GPCR pharmacology using the DiscoveRx HitHunter |
| Luminescence-based assay to detect low levels of cAMP |
| AN 141 |
| ADP Hunter™ Assay for HTS of Kinase Inhibitors using the PHERAstar |
| The ADP Hunter™ assay from DiscoveRx allows for determination of active protein kinases |
| AN 140 |
| High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA |
| The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium. |
| AN 136 |
| LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA |
| Measurement of certified calibration reference materials in comparison to biological controls |
| AN 135 |
| Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader |
| Assessment of the dose-dependent inhibition of temperature-induced protein aggregation by molecular chaperons. |
| AN 132 |
| Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules |
| Thioflavin T aggregation assay to determine antibody stability. |
| AN 131 |
| Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader |
| Screening of target enzymes without need of specific antibodies or radioactive beads |
| AN 130 |
| Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader |
| Detection of mycoplasma in as little as 20 minutes using a bioluminescent assay |
| AN 128 |
| High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader |
| Unfolding transitions of proteins are observed by measuring tryptophan fluorescence |
| AN 124 |
| Identifcation of false positives in an FP Screen |
| Identification of interfering compounds and non specific inhibitors |
| AN 123 |
| Ion channel assay development using Invitrogen's FRET-based voltage sensor probes |
| POLARstar OPTIMA and NOVOstar plate readers are suitable platforms for development of VSP ion channel assays. |
| AN 122 |
| Use of CyDye Fluors for Improved FRET Protease Assays on POLARstar |
| CyDye™ labeled peptides are used as substrates in protease assays |
| AN 119 |
| Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence |
| The gene expression reporter GFP used in FP mode and applied to yeast based genotoxicity assay |
| AN 116 |
| DNA Quantification (Absorbance Mode) |
| The most common method for quantifying DNA samples is by conventional absorbance measurements. |
| AN 113 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence. |
| AN 112 |
| Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood |
| Ultrasensitive analysis of free radicals using a very small blood volume (0.2 µl) |
| AN 111 |
| Chemiluminescence measurement of the generation of reactive oxygen species |
| Very low ROS concentrations in Candida species detected. |
| AN 110 |
| Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy |
| The ATP detection kit is linear through-out kit range |
AN 108 |
| Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants |
| Measurement of ROS scavengers and other antioxidants using the photoprotein of a marine rockboring bioluminescent mollusk. |
AN 107 |
| Rapid functional screening at dopamine receptors by measuring intracellular calcium |
| Using the NOVOstar for calcium measurements of Oregon Green loaded HEK293 cells |
| AN 106 |
| Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans |
| The effect of terbinafine on ROS generation was analyzed in the LUMIstar microplate reader |
| AN 105 |
| Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus |
| The intracellular ROS formation is measured using 2',7'-dichlorfluorescein diacetate (DCFH-DA) to detect hydrogen peroxide (H2O2). |
| AN 104 |
| Intracellular Calcium Assay performed on NOVOstar Microplate Reader |
| Showing the ability of the NOVOstar and its unique pipetting system to screen compounds in 96-well or 384-well format |
| AN 103 |
| Molecular Probes PicoGreen assay performed on the POLARstar OPTIMA microplate reader |
| A standard DNA quantification assay using fluorescence |
NEPHELOstar Plus
| AN 174 |
| Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH's NEPHELOstar |
| Laser nephelometry used for fungal growth determination in presence of antifungal agents |
| AN 133 |
| Measurement of Rheumatoid Factor by NEPHELOstar Microplate Reader |
| Detection and quantification of rheumatoid factors (immunoglobulins Igs) |
| AN 125 |
| Monitoring of Microbial Growth Curves by Laser Nephelometry |
| Correlation between nephelometry and absorbance in microbial growth monitoring |
| AN 117 |
| A fully automated solubility screen in 384-well plate format using nephelometry |
| Kinetic solubility determination of 24 compounds in 75 min |
| AN 114 |
| Nephelometric Test for Determination of Colostrum Quality |
| Quantification of IgG1 in milk and colostrum |
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