The Dual-Luciferase® Reporter (DLR™) Assay System from Promega Corp. provides a luminescent reporter assay to study gene transcription and regulation. The activities of two luciferases are measured sequentially from a single sample reporting the transcription of interest and a transfection control.
Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors, such as in the OPTIMA Series, Omega Series, PHERAstar FS and NOVOstar microplate readers. In the DLR™ Assay System, both reporters yield linear assays with attomole sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.
Dual Luciferase Reaction - Luciferase Assay Reagant II (LAR II) is injected in the first step and the Firefly reaction is started. Stop and Glo® buffer is injected in the second step, which quenches the Firefly reaction and initiates the Renilla reaction
BMG LABTECH has published Application Notes (AN) in cooperation with Promega that demonstrate the use of DLR™ technology with BMG LABTECH microplate readers.
Dual Luciferase Reporter (DLR™) Assay Certification on the Omega Series of Readers (AN 165)
Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon (AN 172)
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega (AN184)
For more information on DLR ratio Evaluation, take a look at the following video:
This video from BMG LABTECH shows Test Protocol Settings for an automated Dual Luciferase kinetic reaction measurement including various result calculations done with the comprehensive Data Analysis Software MARS.
For best results with the Dual-Luciferase® Assay, Promega recommends using a luminometer that has been validated for use with the assay such as the following BMG LABTECH microplate readers:
For more information about DLR™, please see Promega's website: www.promega.com