Cell signaling

No communication and cellular reactions without signaling molecules

Cells not only receive extracellular cues, they also translate them into intracellular reactions. Extracellular signaling molecules comprise growth factors, hormones, ions, neurotransmitters, cytokines and other molecules such as extracellular ATP as well as proteins such as Sclerostin. Our Microplate readers can readily characterize cytokine, hormone, and second messenger changes.

Cellular reaction time covers from a few seconds to several hours. Accordingly, responses must either be triggered and measured instantly, or they need to be observed in living cells over a longer period of time. BMG LABTECH microplate readers provide injectors and a high sampling rate if fast reactions need to be captured. Further, the Atmospheric Control Unit regulates CO2 and O2 levels inside the reading chamber to provide a feel-good atmosphere for long-term cell experiments.

Signaling events rarely come alone, they take place simultaneously or in concert. In order to clearly characterize signaling events, BMG LABTECH multi-mode microplate readers offer the possibility of reading several assays in just one well.

Ion channels

Ion channels

Ions can travel through the membrane using ion channels. Their activity is measured with fluorescent assays and a BMG LABTECH microplate reader.
Reaction catalyzed by kinases: Kinases transfer phosphate groups from ATP to a biomolecule.

Kinases

Kinases phosphorylate proteins and modify their binding characteristics, stability or enzymatic activity. Measure kinase activity or inhibitors on BMG LABTECH plate readers.
The ATP-ADP cycle

ATP

ATP is the fuel of life but is also involved in signaling processes such as the activation of purinergic receptors. Read how to analyze ATP signaling in microplate format.
Illustration of second messengers

Second messengers

The signaling molecules respond to extracellular stimuli. No matter if Ca2+, cAMP, DAG or IP3, live or endpoint, fast or sustained response: our readers measure them all.
Serotonin chemical formula

Hormones

They regulate processes in the organism and their dysregulation leads to diseases. Discover how to measure hormones, receptor interaction, secretion, or regulation.
Different cytokines

Cytokines

Do you need to quantify growth factors, tumor necrosis factor, interleukins, interferons and chemokines? Learn more on the assays and improved methods here.

Resources

Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.

Application notes Blog articles Citations Scientific talks Customer voices Compatible readers
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