LUMIstar Omega for Sensitive Luminescence Detection

Product details Reviews Application notes Citations Technical specifications Accessories Compare readers

LUMIstar Omega

The LUMIstar Omega is a dedicated luminescence plate reader for luminescence measurements, including BRET. It provides highly sensitive readings of all luminescence applications in end-point and kinetic assays. Moreover, a built-in filter wheel and the optional Simultaneous Dual Emission detection make multiple wavelength detection and BRET applications possible.

Features

Flash, glow and BRET assays
Measurements in up to 384-well plates
Top and bottom reading
Incubation up to 45 or 65 °C
Simultaneous Dual Emission
Well scanning with up to 900 data points per well
Injection at point of measurement
Easily integrated into automated systems

Our technologies

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Gas Vent

Incubation and shaking

Path length correction

Reagent injectors

Simultaneous Dual Emission

UV/vis spectrometer

Well-scanning

LUMIstar Omega

Detection Modes

Luminescence (flash and glow) - incl. BRET

Measurement Modes

Top and bottom reading
Endpoint and kinetic
Sequential multi emission
Simultaneous Dual Emission
Ratiometric measurements

Microplate Formats

6 to 384-well plates, user-definable

Microplate Carrier

Robot compatible

Detectors

Low noise photomultiplier tube

Wavelength Selection

Optical filters

Spectral Range

Filters
240 - 740 nm for LUM

Sensitivity

LUM
< 20 amol/well ATP

Read Times

16 s (96), 47 s (384)

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Optical Filters

Emission filter wheel for 8 filters

Optical Path Guides

Top: liquid-filled light guides
Bottom: fiber optics

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing

Power Requirements

100-240 V, 50/60 Hz, max. peak 300 VA

Power Consumption

Instrument off: 0 W
Normal operating conditions: 25 W
Normal operating conditions, incubation set to 37oC: 60 W

Optional Accessories

THERMOstar

Microplate incubator and shaker

Atmospheric Control Unit (ACU)

Actively regulates O₂ and CO₂ 0.1-20%

Stacker

Magazines for up to 50 plates - continuous loading feature

Optical Filters

Emission filters

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
Microplates: white for LUM
96 = 96-well microplates
384 = 384-well microplates

Compare two readers

SPECTROstar Nano

Absorbance plate reader with cuvette port

LUMIstar Omega

Dedicated microplate luminometer

SPECTROstar Omega

Absorbance plate reader with ultrafast UV/vis spectrometer

NEPHELOstar Plus

Microplate nephelometer for light-scattering and turbidity measurements

PHERAstar FSX

Powerful and most sensitive HTS plate reader

CLARIOstar Plus

Most flexible Plate Reader for Assay Development

FLUOstar Omega

Filter-based multi-mode microplate reader

VANTAstar

Flexible microplate reader with simplified workflows

LUMIstar Omega

Detection Modes

Luminescence (flash and glow) - incl. BRET

Measurement Modes

Top and bottom reading
Endpoint and kinetic
Sequential multi emission
Simultaneous Dual Emission
Ratiometric measurements

Microplate Formats

6 to 384-well plates, user-definable

Microplate Carrier

Robot compatible

Detectors

Low noise photomultiplier tube

Wavelength Selection

Optical filters

Spectral Range

Filters
240 - 740 nm for LUM

Sensitivity

LUM
< 20 amol/well ATP

Read Times

16 s (96), 47 s (384)

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Optical Filters

Emission filter wheel for 8 filters

Optical Path Guides

Top: liquid-filled light guides
Bottom: fiber optics

Reagent Injection

Power Requirements

100-240 V, 50/60 Hz, max. peak 300 VA

Power Consumption

Instrument off: 0 W
Normal operating conditions: 25 W
Normal operating conditions, incubation set to 37oC: 60 W

Optional Accessories

THERMOstar

Microplate incubator and shaker

Atmospheric Control Unit (ACU)

Actively regulates O₂ and CO₂ 0.1-20%

Stacker

Magazines for up to 50 plates - continuous loading feature

Optical Filters

Emission filters

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
Microplates: white for LUM
96 = 96-well microplates
384 = 384-well microplates

LUMIstar Omega

Dedicated microplate luminometer

Detection modes

LUMIstar Omega

Features

Incubation and shaking