For the maintenance of ordered production systems, it is essential to ensure the consistent and accurate quality of the production processes and consequently the final product. This applies in particular to products which are intended for human use such as foods, drugs, and medicinal products, or products applied to life stock or plants, since contaminants or falsely administered concentrations can lead to health issues or disease. The quality of a product is made up of various quality parameters. It is important to ensure consistent purity, composition and quantity. Microplate readers offer various solutions for determining these quality parameters.
The determination of contaminants and impurities in a sample is an important prerequisite for many downstream applications of products. Such impurities can range from undesired side products that can arise in a production process to microbial contaminations and toxins that can accumulate in a product. Ordered production systems often also include purification procedures. However, these purification processes themselves also need to be validated to ensure product purity, since such procedures can be inefficient or even introduce additional or new contaminants. Microplate reader-based methods, for example for the analysis of DNA purity or the detection of endotoxins, are the ideal tools to accomplish this task since they combine sensitivity and minimal sample volumes with the ability to achieve high sample throughput. Microplate readers are used frequently to identify mycoplasma contaminations in cell culture with assay kits like MycoAlert®.
But product purity alone isn´t the only measure for performing adequate quality control. Even if a product is pure and free of contaminants it should have a consistent composition and stability. The application note Antibody Aggregation on a Plate Reader, presents an example to test the stability of manufactured antibodies.. Drugs and biologicals, in particular have to be administered precisely to induce their effect to a targeted extent, since over-dosage can quickly lead to undesirable side effects. This principle also applies to the field of life sciences, as experimental results can only be interpreted correctly if researchers know the accurate composition and concentrations of their samples. Many methods, such as the quantification of antioxidant capacity, protein quantification, reporter assays or ELISAs can be performed on microplate readers, offering cost-effective and accurate solutions for quality control testing.
Search our resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Are you looking for a device to measure sample`s biotoxicity with Aliivibrio fischeri? BMG LABTECH's luminometers fulfil this task and offer additional advantages over the classic cuvette-based analysis.
Mycoplasma contamination is a serious problem in cell culture labs. The MycoAlert™ luminescence test allows quick and easy detection of mycoplasmas in cell cultures to prevent their spread.
Quality control of consumables is always crucial, even for distilled spirits! See how UV-Vis scanning and `fingerprint´ detection can be used for their quality testing and authentication.
Standard brewing quality control analyses include the test for free amino nitrogen (FAN). The ninhydrin-based FAN assay for cuvettes was scaled down to run in microplate formats.
Monitoring of the metalation of a porphyrin with Zn2+ as a function of solvent by the acquisition of absorbance spectra over time on a BMG LABTECH microplate reader.
Changes in intrinsic tryptophan fluorescence can be used to investigate protein-ligand interactions. In this case the binding of an antibody to its antigen was analysed.
Gene reporter assays are sensitive and specific tools to study the regulation of gene expression. Learn about the different options available, their uses, and the benefits of running these types of assays on microplate readers.
You want to make sure that your cell culture is free of mycoplasma? BMG LABTECH microplate readers are the perfect measurement platform to read MycoAlert® mycoplasma detection kits.
Endotoxins are highly toxic molecules that lead to sepsis even in small amounts. This article explains what endotoxins are and how microplate readers can be used for their detection.
The LAL assay ensures that sterile pharmaceutical products and medical devices are safe for human use. This test can be run efficiently and in high throughput on a plate reader.
Read here about the various threats associated with mycoplasma contamination and find out about methods to prevent, detect, and eliminate mycoplasma in your cell culture.
Read here how absorbance-based methods on the SPECTROstar Nano can be used for quality testing of alcohol.
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