SPECTROstar Omega マイクロプレートリーダー

評価

Very easy to set up and fully automatised

Highly reproducible results and very easy to use

★★★★☆

4.7/5.0 (3)

Rate

All reviews

製品詳細レビューアプリケーションノート引用技術仕様付属品リーダーを比較

SPECTROstar Omega

SPECTROstar Omegaは、フルスキャン測定だけでなく、最大８つまで指定した波長を完全同時に測定することが可能です。SPECTROstar Omegaは、吸光度専用機から蛍光測定・発光測定が可能なマルチモードリーダーにアップグレードが可能です。さらには二波長同時測定・試薬ディスペンサのオプションも選択できます。

特徴

高速スペクトル吸光度測定
1536wellプレートまで対応
45℃もしくは65℃までの温度制御
各種シェイキング
ウェル毎に最大900ポイントでウェルスキャニング
試薬注入と検出を同時に行う高精度インジェクター（2chまで)
微量測定可能

当社の技術

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

UV/vis spectrometer

BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

Well-scanning

For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.

Gas Vent

Incubation and shaking

Path length correction

Reagent injectors

UV/vis spectrometer

Well-scanning

SPECTROstar Omega

Detection Modes

UV/vis absorbance spectra

Measurement Modes

Endpoint and kinetic
Spectral scanning (absorbance)
Well scanning

Microplate Formats

6 to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp

Detectors

CCD Spectrometer

Wavelength Selection

UV/Vis Absorbance Spectrometer
Full spectra or 8 distinct wavelengths in < 1 sec/well

Spectral Range

Spectrometer
220 - 1000 nm; wavelength precision: ≤ 0.5 nm

Sensitivity

Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD

Read Times

Full spectrum from 220 to 1000 nm in less than 1 sec/well

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing

Power Requirements

100-240 V, 50/60 Hz, max. peak 300 VA

Power Consumption

Instrument off: 0 W
Normal operating conditions: 25 W
Normal operating conditions, incubation set to 37oC: 60 W

Optional Accessories

LVis Plate

Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples

Quality control internal standards (optional): four NIST-traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates

THERMOstar

Microplate incubator and shaker

Atmospheric Control Unit (ACU)

Actively regulates O₂ and CO₂ 0.1-20%

Stacker

Magazines for up to 50 plates - continuous loading feature

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

Compare two readers

SPECTROstar Nano

Absorbance plate reader with cuvette port

LUMIstar Omega

Dedicated microplate luminometer

SPECTROstar Omega

Absorbance plate reader with ultrafast UV/vis spectrometer

NEPHELOstar Plus

Microplate nephelometer for light-scattering and turbidity measurements

PHERAstar FSX

Powerful and most sensitive HTS plate reader

CLARIOstar Plus

Most flexible Plate Reader for Assay Development

FLUOstar Omega

Filter-based multi-mode microplate reader

VANTAstar

Flexible microplate reader with simplified workflows

SPECTROstar Omega

Detection Modes

UV/vis absorbance spectra

Measurement Modes

Endpoint and kinetic
Spectral scanning (absorbance)
Well scanning

Microplate Formats

6 to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp

Detectors

CCD Spectrometer

Wavelength Selection

UV/Vis Absorbance Spectrometer
Full spectra or 8 distinct wavelengths in < 1 sec/well

Spectral Range

Spectrometer
220 - 1000 nm; wavelength precision: ≤ 0.5 nm

Sensitivity

Read Times

Full spectrum from 220 to 1000 nm in less than 1 sec/well

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Reagent Injection

Power Requirements

100-240 V, 50/60 Hz, max. peak 300 VA

Power Consumption

Instrument off: 0 W
Normal operating conditions: 25 W
Normal operating conditions, incubation set to 37oC: 60 W

Optional Accessories

LVis Plate

Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples

Quality control internal standards (optional): four NIST-traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates

THERMOstar

Microplate incubator and shaker

Atmospheric Control Unit (ACU)

Actively regulates O₂ and CO₂ 0.1-20%

Stacker

Magazines for up to 50 plates - continuous loading feature

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

SPECTROstar Omega

フルスペクトル吸光度測定専用マイクロプレートリーダー

検出モード

評価

Very easy to set up and fully automatised

SPECTROstar Omega

特徴

Incubation and shaking