VANTAstar

Fluorescence intensity (incl. FRET)
Fluorescence polarization
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra

Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning

6- to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Robot compatible

High energy xenon flash lamp

Low-noise photomultiplier tube
CCD spectrometer

Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators

Optical filters:
Ex and em slides hold up to 5 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well

Filters
FI, LUM, TRF, FP, TR-FRET: 240 - 740 nm

LVF Monochromators™
FI, LUM: 320 - 740 nm

Linear Variable Dichroic
340 - 740 nm

Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm

FI filters
(top)
< 0.5 pM (< 10 amol/well FITC, 384sv, 20 μL).

FI filters
(bottom)
< 2.5 pM (< 125 amol/well FITC, 384g, 50 μL).

FI monochromator
(top)
< 0.8 pM (< 16 amol/well FITC, 384sv, 20 μL).

FI monochromator
(bottom)
< 3.5 pM (< 175 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.

FP
< 1.2 mP SD at 1 nM FITC (384sv, 20 μL).

HTRF^®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 700% (High Calibrator).
Delta F > 25% (Low Calibrator).

TRF
< 30 fM europium (< 2.4 amol/well, 384, 80 μL).

LUM
< 0.8 pM (< 15 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.

ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.

Flying mode (1 flash)
9 sec (96), 16 sec (384)

Linear, circular and double-circular with user-definable time and speed

+4 °C above ambient up to 45 °C or 60 °C

Minimum condensation concept: the upper heating plate of the incubation chamber operates at +0.5 °C than the lower plate, heated optic system

Multi-user reader control (SMART Control and Voyager) and data analysis software (MARS). 

FDA 21 CFR Part 11 compliance available with SMART Control and MARS.

Integrated fluorophore library.

Width: 35 cm, depth: 52 cm, height: 36 cm; weight: 27 kg

Excitation and emission slides for up to 4 filters each for monochoromator units (VANTAstar), and for up to 5 filters each for filter-only units (VANTAstar F)

Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

Automatic focal height adjustment (0.1 mm resolution)

Up to 2 reagent injectors
Individual injection volumes for each well: 3 to 500 μL (optionally up to 1 mL)
Variable injection speed up to 420 μL/s
Reagent back flushing
Heater and stirrer plate
Dimensions: width: 13 cm, depth: 31 cm, height: 26 cm; weight: 6 kg

• External power supply
• Input Voltage: 100 - 240 V AC, 50 - 60 Hz, auto ranging.
• Input current ≤ 2.0 A.
• Output Voltage: 24 V DC / 6.25 A

• Standby ≤ 0.15 W
• Idle mode 10 W
• Normal operating conditions 30 W   
• Maximum 150 W

Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples

Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates

Microplate incubator and shaker

Actively regulates O₂ and CO₂ - 1-20%

Excitation and emission filters

Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available

Please contact your local representative for more information

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates

The VANTAstar is for research use only.