NEPHELOstar Plus

Nefelômetro de microplacas para medições de dispersão de luz e turbidez

O primeiro e único nefelômetro de microplacas baseadas em laser do mundo foi desenvolvido para atender às demandas de alto rendimento para telas de solubilidade de compostos rápidos. O NEPHELOstar^® Plus detecta partículas insolúveis em amostras líquidas através da medição da luz dispersa para frente e pode ser usado para medições de turbidez, triagem de solubilidade de drogas, cinética de ligação de proteína, crescimento microbiano e controle de processo de petróleo.

Detalhes do produto Avaliações Notas de aplicação Citações Especificações técnicas Acessórios Comparar leitores

NEPHELOstar Plus

O NEPHELOstar Plus é um nefelômetro de microplacas com um diodo laser de alta intensidade. Pode ser até trinta vezes mais sensível do que os leitores de densidade óptica de transmissão tradicionais que medem a redução na luz direta que passa por um poço de amostra. Graças à sua velocidade, flexibilidade e desempenho, o NEPHELOstar Plus permite que mais aplicações sejam adaptadas à nefelometria laser baseada em microplacas do que nunca.

Características

Intensidade do laser ajustável e diâmetro do feixe
Medição em formato de microplaca de até 384 poços
Incubação de até 45 °C
Tremido linear, orbital e duplo orbital
Injetores de alta precisão com injeção e detecção simultânea de reagentes
Microplaca Stacker e robô compatível

As nossas tecnologias

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Automation

Automation is a key feature in high-throughput screening. BMG LABTECH microplate readers have been successfully integrated into various robotic systems. Small footprint, multiple robotic software interfaces and an automation-friendly plate carrier guarantee an easy integration into all leading robotic platforms. All plate readers can be controlled through ActiveX or DDE interfaces. Moreover, the ASCII data output is the most versatile in the industry and works with most LIMS and automation platforms.

Gas Vent

BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.

Reagent injectors

Automation

Gas Vent

Incubation and shaking

Reagent injectors

NEPHELOstar Plus

Detection Modes

Nephelometry (light scattering)

Measurement Modes

Endpoint and kinetic

Microplate Formats

Up to 384-well plates

Microplate Carrier

Robot compatible

Light Sources

Self-monitoring laser diode
Wavelength 635±10 nm
Stability <0.2% deviation
Lifetime 20,000 hours
Output: 1 mW
Selectable beam width: 1.5 to 3.5 mm
Selectable intensity 0-100%
Scattering angle: detects up to 80° full cone angle

Detectors

Side window photodiode detector

Wavelength Selection

Photodiode
Wavelength 635±10 nm

Sensitivity

Depending on particle size and liquid properties
Silica detection (particle size 0.5 to 10 µm) 800 nM
Dynamic Range: 5 decades
Maximum count rate (2,000,000 Relative Nephelometry Units (RNUs) per second)

Read Times

Depend on assay conditions and liquid surface stability
Shortest times possible: 16 s (96), 47 s (384)

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 60 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 32 cm; weight: 25 kg

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing

Power Requirements

Input Voltage: 100-240 V, 50/60 Hz, max peak 300 VA

Power Consumption

Instrument off: 0 W
Normal operating conditions: 15 W
Normal operating conditions, incubation set to 37oC: 50 W

Optional Accessories

THERMOstar

Microplate incubator and shaker

Atmospheric Control Unit (ACU)

Actively regulates O2 and CO2 0.1-20%

Stacker

Magazines for up to 50 plates - continuous loading feature

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

Compare dois leitores

Compare two readers

SPECTROstar Nano

Absorbance plate reader with cuvette port

LUMIstar Omega

Dedicated microplate luminometer

SPECTROstar Omega

Absorbance plate reader with ultrafast UV/vis spectrometer

NEPHELOstar Plus

Microplate nephelometer for light-scattering and turbidity measurements

PHERAstar FSX

Powerful and most sensitive HTS plate reader

CLARIOstar Plus

Most flexible Plate Reader for Assay Development

FLUOstar Omega

Filter-based multi-mode microplate reader

VANTAstar

Flexible microplate reader with simplified workflows

NEPHELOstar Plus

Detection Modes

Nephelometry (light scattering)

Measurement Modes

Endpoint and kinetic

Microplate Formats

Up to 384-well plates

Microplate Carrier

Robot compatible

Light Sources

Detectors

Side window photodiode detector

Wavelength Selection

Photodiode
Wavelength 635±10 nm

Sensitivity

Read Times

Depend on assay conditions and liquid surface stability
Shortest times possible: 16 s (96), 47 s (384)

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 60 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 32 cm; weight: 25 kg

Reagent Injection

Power Requirements

Input Voltage: 100-240 V, 50/60 Hz, max peak 300 VA

Power Consumption

Instrument off: 0 W
Normal operating conditions: 15 W
Normal operating conditions, incubation set to 37oC: 50 W

Optional Accessories

THERMOstar

Microplate incubator and shaker

Atmospheric Control Unit (ACU)

Actively regulates O2 and CO2 0.1-20%

Stacker

Magazines for up to 50 plates - continuous loading feature

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

NEPHELOstar Plus

Nefelômetro de microplacas para medições de dispersão de luz e turbidez

Modos de detecção

NEPHELOstar Plus

Características

Reagent injectors

Automation

Gas Vent

Incubation and shaking

Reagent injectors

Automation

Gas Vent

Incubation and shaking

Reagent injectors

Improving throughput for assessing nephelometric turbidity units (NTUs) using the NEPHELOstar® Plus

Nephelometric monitoring growth of Candida albicans using BMG LABTECH's NEPHELOstar Plus

Measuring of rheumatoid factors by the NEPHELOstar Plus microplate reader

Monitoring of microbial growth curves by laser nephelometry

A fully automated kinetic solubility screen in 384-well plate format using nephelometry

Effect of Denosumab on Bone Health, Vascular Calcification, and Health-Related Quality of Life in Hemodialysis Patients with Osteoporosis: A Prospective Observational Study

Outline and Background for the EU-OS Solubility Prediction Challenge

High-throughput 61000765301 methodology for qualitative determination of aqueous solubility of chemical libraries

Differential associations of fetuin-A and calcification propensity with cardiovascular events and subsequent mortality in patients undergoing hemodialysis

A method to determine antifungal activity in seed exudates by 61000765301

Discovery, Optimization, and Evaluation of Novel Pyridin-2(1H)-one Analogues as Potent TRK Inhibitors for Cancer Treatment

Para mais efeitos: Acessórios para NEPHELOstar Plus

THERMOstar

Microplate Stacker

Atmospheric Control Unit

Compare dois leitores

Compare two readers

SPECTROstar Nano

LUMIstar Omega

SPECTROstar Omega

NEPHELOstar Plus

PHERAstar FSX

CLARIOstar Plus

FLUOstar Omega

VANTAstar